YellowSub PCR Additive 10x1 ml

Article number

FL 120

YellowSub PCR Additive

Yellow Sub^TM is suitable for many enzymatic reactions. It increases the yield and specificity of your PCR^® - and saves time. Just add the specially formulated 10 x Yellow Sub^TM to your PCR^®-mix. Yellow SubTM also contains a loading buffer and dye, so after the reaction you can apply the PCR^®-mix directly on your agarose gel without adding any further solution. The yellow dye migrates at about 40 bp leaving even short bands easily visible. Yellow Sub^TM has been successfully tested in PCR^® applications using different Taq Polymerases and also other thermostable polymerases like Pfu, Tth and Pfx. Furthermore, Yellow Sub is even compatible with many restriction enzymes and is specially suited for restriction digest screening procedures. Yellow Sub^TM is successfully used in many laboratories and quoted in several recent studies.

Product Features of Yellow Sub^TM

• Increased yield in PCR^® applications
• Enhanced specificity in PCR® applications
• Contains the loading buffer and dye - the reaction mix is applied directly to the agarose gel
• Yellow dye migrates at 40 bp - even short PCR®-bands are easily visible
• Compatible with all tested thermostable polymerases
• Compatible with DNA-purification protocols (glass milk or Qiagen spin columns). PCR® fragments can be used in all regular applications like cloning or restriction digests

Publications

1. Pre-PCR Gel-Loading Buffer that Increases Specificity
Haack, K. Vizuete-Forster, M.
BioTechniques. 2000; 29:684-688.

2. Polymorphic Sex Testing
International Patent Application No. PCT/GB2002/003920; Filing Date: 27.08.2002

3. Tumor cells escape suicide gene therapy by genetic and epigenetic instability
Frank, O. et al.
Blood. 2004; Dec 1;104(12):3543-9

4. Epidemiological study of the prevalence of Babesia divergens in a
veterinary practice in the mid-east of France
Devosa, J. and Geysen, D.
Veterineray Parasitology. 2004; 125(3-4): 237-249.

5. Taeniasis and Cysticercosis in a Selected Group of Inhabitants from a Mountainous Province in Nother Vietnam
ITMA – MSTAH thesis, No 56, 2006.

6. Quantification of DNA methylation in electrofluidics chips (Bio-COBRA).
Brena RM, Auer H, Kornacker K, Plass C,
Nature Protocols. 2006;1(1):52-8.

7. Development and validation of a PCR-RFLP test to identify African Rhipicephalus (Boophilus) ticks
Laetitia Lempereur, Dirk Geysen and Maxime Madder
Acta Tropica, Volume 114, Issue 1, April 2010: 55-58

8. Evaluation of a Real-Time PCR Test for the Detection and Discrimination of Theileria Species in the African Buffalo (Syncerus caffer)
Mamohale E. Chaisi et al.
PLOS ONE: October 17, 2013

9. MALDI-TOF mass spectrometry fingerprinting: A diagnostic tool to differentiate dematiaceous fungi Stachybotrys chartarum and Stachybotrys chlorohalonata 
Maike Gruenwald et al.
Journal of Microbiological Methods 115 (2015) 83–88

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